MersV1, Main, Exploration, bibRecord, 003B24

Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes

Identifieur interne : 003B24 ( Main/Exploration ); précédent : 003B23; suivant : 003B25

Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes

Auteurs : Kimberly H. Abramo [États-Unis] ; J. Bruce Pitner [États-Unis] ; Linda B. Mcgown [États-Unis]

Source :

Biospectroscopy [ 1075-4261 ] ; 1998.

RBID : ISTEX:96678992D4CBAF5486AF7444019CB99E3E2BA55C

Descripteurs français

KwdFr :
- ADN simple brin (), ADN simple brin (métabolisme), Analyse de Fourier, Benzoxazoles (), Benzoxazoles (métabolisme), Cinétique, Colorants fluorescents (), Colorants fluorescents (métabolisme), Conformation d'acide nucléique, Dichroïsme circulaire, Fixation compétitive, Ligands, Polarisation de fluorescence, Quinoléines (), Quinoléines (métabolisme), Relation structure-activité, Spectrométrie de fluorescence, Séquence nucléotidique.
MESH :
- métabolisme : ADN simple brin, Benzoxazoles, Colorants fluorescents, Quinoléines.
- ADN simple brin, Analyse de Fourier, Benzoxazoles, Cinétique, Colorants fluorescents, Conformation d'acide nucléique, Dichroïsme circulaire, Fixation compétitive, Ligands, Polarisation de fluorescence, Quinoléines, Relation structure-activité, Spectrométrie de fluorescence, Séquence nucléotidique.

English descriptors

Abstract

Interactions between short single‐stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G‐quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO‐pro‐1 iodide (YO) and YOYO‐1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G‐quartet forming ligands, a 20‐mer of sequence 5′‐GGTTTT‐GGTTTTGGTTTTGG‐3′, shows distinctly different interactions from the other three ligands, all of which are 15‐mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single‐stranded DNA. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 27–35, 1998

Url:

https://api.istex.fr/ark:/67375/WNG-8B6PJ3X5-V/fulltext.pdf

DOI: 10.1002/(SICI)1520-6343(1998)4:1<27::AID-BSPY3>3.0.CO;2-P

Affiliations:

Le document en format XML

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</author>
<author><name sortKey="Pitner, J Bruce" sort="Pitner, J Bruce" uniqKey="Pitner J" first="J. Bruce" last="Pitner">J. Bruce Pitner</name>
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<author><name sortKey="Mcgown, Linda B" sort="Mcgown, Linda B" uniqKey="Mcgown L" first="Linda B." last="Mcgown">Linda B. Mcgown</name>
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<placeName><region type="state">Caroline du Nord</region>
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<term>Benzoxazoles (chemistry)</term>
<term>Benzoxazoles (metabolism)</term>
<term>Binding, Competitive</term>
<term>Circular Dichroism</term>
<term>DNA, Single-Stranded (chemistry)</term>
<term>DNA, Single-Stranded (metabolism)</term>
<term>Fluorescence Polarization</term>
<term>Fluorescent Dyes (chemistry)</term>
<term>Fluorescent Dyes (metabolism)</term>
<term>Fourier Analysis</term>
<term>Kinetics</term>
<term>Ligands</term>
<term>Nucleic Acid Conformation</term>
<term>Quinolines (chemistry)</term>
<term>Quinolines (metabolism)</term>
<term>Spectrometry, Fluorescence</term>
<term>Structure-Activity Relationship</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN simple brin ()</term>
<term>ADN simple brin (métabolisme)</term>
<term>Analyse de Fourier</term>
<term>Benzoxazoles ()</term>
<term>Benzoxazoles (métabolisme)</term>
<term>Cinétique</term>
<term>Colorants fluorescents ()</term>
<term>Colorants fluorescents (métabolisme)</term>
<term>Conformation d'acide nucléique</term>
<term>Dichroïsme circulaire</term>
<term>Fixation compétitive</term>
<term>Ligands</term>
<term>Polarisation de fluorescence</term>
<term>Quinoléines ()</term>
<term>Quinoléines (métabolisme)</term>
<term>Relation structure-activité</term>
<term>Spectrométrie de fluorescence</term>
<term>Séquence nucléotidique</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Benzoxazoles</term>
<term>DNA, Single-Stranded</term>
<term>Fluorescent Dyes</term>
<term>Quinolines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Benzoxazoles</term>
<term>DNA, Single-Stranded</term>
<term>Fluorescent Dyes</term>
<term>Quinolines</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN simple brin</term>
<term>Benzoxazoles</term>
<term>Colorants fluorescents</term>
<term>Quinoléines</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Absorbance</term>
<term>Absorption spectra</term>
<term>Anisotropy</term>
<term>Anisotropy measurements</term>
<term>Base Sequence</term>
<term>Base composition</term>
<term>Binding constants</term>
<term>Binding interactions</term>
<term>Binding modes</term>
<term>Binding selectivity</term>
<term>Binding stoichiometries</term>
<term>Binding stoichiometry</term>
<term>Binding, Competitive</term>
<term>Biosas</term>
<term>Circular Dichroism</term>
<term>Circular dichroism</term>
<term>Circular dichroism spectra</term>
<term>Conformational differences</term>
<term>Dichroism</term>
<term>Duke university</term>
<term>Dye</term>
<term>Emission beam</term>
<term>Emission wavelength</term>
<term>Environmental health sciences</term>
<term>Equilibrium binding constants</term>
<term>Equilibrium constants</term>
<term>Excitonic</term>
<term>Fluorescence Polarization</term>
<term>Fluorescence anisotropy</term>
<term>Fourier Analysis</term>
<term>Greater degree</term>
<term>Homogeneous base composition</term>
<term>Human virus type</term>
<term>Indicator dyes</term>
<term>Individual ligand</term>
<term>Intramolecular</term>
<term>Intramolecular excitonic</term>
<term>John wiley sons</term>
<term>Kinetics</term>
<term>Lifetime distributions</term>
<term>Ligand</term>
<term>Ligand concentrations</term>
<term>Ligands</term>
<term>Mcgown</term>
<term>Nals</term>
<term>National institute</term>
<term>National institutes</term>
<term>Nucleic Acid Conformation</term>
<term>Other hand</term>
<term>Other ligands</term>
<term>Oxazole</term>
<term>Photophysical properties</term>
<term>Pitner</term>
<term>Quantum yields</term>
<term>Santa clara</term>
<term>Single strands</term>
<term>Spec</term>
<term>Spectrometry, Fluorescence</term>
<term>Spectronics instruments</term>
<term>Spectroscopic studies</term>
<term>Stoichiometry</term>
<term>Structure-Activity Relationship</term>
<term>Target molecules</term>
<term>Toxicology training grant program fellowship</term>
<term>Uorescence</term>
<term>Uorescent dyes</term>
<term>Yoyo</term>
<term>Yoyo peak</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>ADN simple brin</term>
<term>Analyse de Fourier</term>
<term>Benzoxazoles</term>
<term>Cinétique</term>
<term>Colorants fluorescents</term>
<term>Conformation d'acide nucléique</term>
<term>Dichroïsme circulaire</term>
<term>Fixation compétitive</term>
<term>Ligands</term>
<term>Polarisation de fluorescence</term>
<term>Quinoléines</term>
<term>Relation structure-activité</term>
<term>Spectrométrie de fluorescence</term>
<term>Séquence nucléotidique</term>
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<front><div type="abstract" xml:lang="en">Interactions between short single‐stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G‐quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO‐pro‐1 iodide (YO) and YOYO‐1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G‐quartet forming ligands, a 20‐mer of sequence 5′‐GGTTTT‐GGTTTTGGTTTTGG‐3′, shows distinctly different interactions from the other three ligands, all of which are 15‐mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single‐stranded DNA. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 27–35, 1998</div>
</front>
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<name sortKey="Mcgown, Linda B" sort="Mcgown, Linda B" uniqKey="Mcgown L" first="Linda B." last="Mcgown">Linda B. Mcgown</name>
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Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes

Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes

Source :

Descripteurs français

English descriptors

Abstract

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